############# Model summary Number of compounds: 3316 Number of reactions: 3402 Number of genes: 5372 Ratio rxn with genes/rxns: 96.00235155790712% ############# Analyzing targets #Topological analysis Number of targets: 50 #Flux Balance Analysis CPD__45__8117_c // CPD-8117_c 0.0 NULL LEU_c // LEU_c 659.7593582887761 positive DOCOSANOATE_c // DOCOSANOATE_c 0.0 NULL CIT_c // CIT_c 1000.0 positive GLC_c // GLC_c 1000.0 positive CPD__45__8120_c // CPD-8120_c 0.0 NULL PALMITATE_c // PALMITATE_c 213.4146341463416 positive SER_c // SER_c 1000.0 positive OLEATE__45__CPD_c // OLEATE-CPD_c 194.4444444444445 positive GLT_c // GLT_c 1000.0 positive CPD__45__12653_c // CPD-12653_c 0.0 NULL L__45__ALPHA__45__ALANINE_c // L-ALPHA-ALANINE_c 1000.0 positive LINOLENIC_ACID_c // LINOLENIC_ACID_c 0.0 NULL HIS_c // HIS_c 365.38461538461553 positive ARG_c // ARG_c 374.99999999999983 positive VAL_c // VAL_c 817.923898531375 positive SUC_c // SUC_c 1000.0 positive PHE_c // PHE_c 723.6842105263164 positive GLUTATHIONE_c // GLUTATHIONE_c 419.31216931216943 positive THREO__45__DS__45__ISO__45__CITRATE_c // THREO-DS-ISO-CITRATE_c 1000.0 positive 5Z8Z11Z14Z17Z__45__EICOSAPENTAENOATE_c // 5Z8Z11Z14Z17Z-EICOSAPENTAENOATE_c 222.2222222222222 positive L__45__ASPARTATE_c // L-ASPARTATE_c 1000.0 positive TYR_c // TYR_c 949.9999999999998 positive LINOLEIC_ACID_c // LINOLEIC_ACID_c 203.4883720930231 positive THR_c // THR_c 1000.0 positive CPD__45__8121_c // CPD-8121_c 0.0 NULL CPD__45__9247_c // CPD-9247_c 0.0 NULL CYS_c // CYS_c 524.8344370860931 positive STEARIC_ACID_c // STEARIC_ACID_c 186.17021276595761 positive HOMO__45__SER_c // HOMO-SER_c 1000.0 positive LYS_c // LYS_c 504.21810699588474 positive ARACHIDONIC_ACID_c // ARACHIDONIC_ACID_c 213.4146341463429 positive TRP_c // TRP_c 395.83333333333337 positive ILE_c // ILE_c 641.8281822943948 positive GLY_c // GLY_c 1000.0 positive CPD__45__9245_c // CPD-9245_c 224.35897435897394 positive 4__45__AMINO__45__BUTYRATE_c // 4-AMINO-BUTYRATE_c 999.1071428571429 positive GLYCOLLATE_c // GLYCOLLATE_c 999.9999999999999 positive ASN_c // ASN_c 980.0000000000041 positive MANNITOL_c // MANNITOL_c 1000.0 positive Eicosadienoates_c // Eicosadienoates_c 0.0 NULL MET_c // MET_c 403.7740899357701 positive L__45__ORNITHINE_c // L-ORNITHINE_c 580.5882352941175 positive PRO_c // PRO_c 999.9999999999999 positive GLYCEROL_c // GLYCEROL_c 1000.0 positive GLN_c // GLN_c 881.5789473684209 positive CPD__45__7836_c // CPD-7836_c 250.0 positive AMMONIUM_c // AMMONIUM_c 998.5311398354884 positive GLYCERATE_c // GLYCERATE_c 1000.0 positive ARACHIDIC_ACID_c // ARACHIDIC_ACID_c 165.0943396226416 positive ############# Computing optimization Testing reaction biomass_rxn Growth rate: 3.556872290882731 Status: optimal IN FLUXES OUT FLUXES OBJECTIVES ------------------ --------------- ----------------- PROTON_e 1e+03 BIOMASS_e 3.56 biomass_rxn 3.56 WATER_e 1e+03 Light_e 500 NITRATE_e 429 SULFATE_e 2.13 FVA analysis: Blocked reactions: 2329 Essential reactions: 46 HISTPRATPHYD__45__RXN DIHYDROXYISOVALDEHYDRAT__45__RXN RXN__45__14014 Transport_BIOMASS BRANCHED__45__CHAINAMINOTRANSFERVAL__45__RXN ORNITHINE__45__CYCLODEAMINASE__45__RXN DIHYDRODIPICSYN__45__RXN HISTCYCLOHYD__45__RXN Exchange_BIOMASS 2__45__MEBUCOA__45__FAD__45__RXN IGPSYN__45__RXN HOMOSERDEHYDROG__45__RXN GLYOXYLATE__45__REDUCTASE__45__NADP__43____45__RXN SHIKIMATE__45__5__45__DEHYDROGENASE__45__RXN ADENYLOSUCCINATE__45__SYNTHASE__45__RXN TIGLYLCOA__45__HYDROXY__45__RXN 1__46__1__46__1__46__178__45__RXN SHIKIMATE__45__KINASE__45__RXN biomass_rxn DAHPSYN__45__RXN RXN__45__7790 RXN__45__7737 METHYLACETOACETYLCOATHIOL__45__RXN 3__45__DEHYDROQUINATE__45__DEHYDRATASE__45__RXN ANTHRANSYN__45__RXN 2__46__5__46__1__46__19__45__RXN DIAMINOPIMEPIM__45__RXN TransportSeed__45__SULFATE ACETOLACTREDUCTOISOM__45__RXN BRANCHED__45__CHAINAMINOTRANSFERILEU__45__RXN IMPCYCLOHYDROLASE__45__RXN SULFATE__45__ADENYLYLTRANS__45__RXN ASPARTATE__45__SEMIALDEHYDE__45__DEHYDROGENASE__45__RXN RXN__45__13158 GLUTDECARBOX__45__RXN 3__45__DEHYDROQUINATE__45__SYNTHASE__45__RXN DIAMINOPIMDECARB__45__RXN GLUTAMINESYN__45__RXN PRAISOM__45__RXN BRANCHED__45__CHAINAMINOTRANSFERLEU__45__RXN PRTRANS__45__RXN CHORISMATE__45__SYNTHASE__45__RXN HISTAMINOTRANS__45__RXN ExchangeSeed__45__SULFATE CHORISMATEMUT__45__RXN ASPARTATEKIN__45__RXN Essential genes: 21 30/30 compounds with positive flux 0/30 compounds without flux LYS_c // LYS_c 1000.0 positive ASN_c // ASN_c 1000.0 positive GLN_c // GLN_c 82.39055582882429 positive GLYCEROL_c // GLYCEROL_c 712.5506072874487 positive PHE_c // PHE_c 1000.0000000000003 positive THR_c // THR_c 999.9999999999998 positive MET_c // MET_c 999.9999999999997 positive CIT_c // CIT_c 126.77151333947933 positive CYS_c // CYS_c 999.9999999999997 positive GLT_c // GLT_c 123.78426171529617 positive GLY_c // GLY_c 394.7368421052632 positive GLYCERATE_c // GLYCERATE_c 1000.0 positive GLYCOLLATE_c // GLYCOLLATE_c 999.999999999998 positive PRO_c // PRO_c 740.7407407407406 positive SER_c // SER_c 437.81094527363194 positive HIS_c // HIS_c 999.9999999999998 positive ILE_c // ILE_c 1000.0000000000014 positive L__45__ALPHA__45__ALANINE_c // L-ALPHA-ALANINE_c 48.52130325814537 positive L__45__ORNITHINE_c // L-ORNITHINE_c 999.9999999999981 positive LEU_c // LEU_c 999.9999999999999 positive MANNITOL_c // MANNITOL_c 3.8174710021421 positive VAL_c // VAL_c 962.2634100369119 positive 4__45__AMINO__45__BUTYRATE_c // 4-AMINO-BUTYRATE_c 1000.0000000000007 positive ARG_c // ARG_c 1000.0000000000001 positive GLC_c // GLC_c 1000.0 positive L__45__ASPARTATE_c // L-ASPARTATE_c 200.0 positive SUC_c // SUC_c 999.9999999999998 positive THREO__45__DS__45__ISO__45__CITRATE_c // THREO-DS-ISO-CITRATE_c 261.1939648639612 positive TRP_c // TRP_c 1000.0 positive TYR_c // TYR_c 1000.0 positive