Difference between revisions of "CPD-10792"
(Created page with "== description == == Automatic reconstruction with [http://aureme.genouest.org AuReMe] == Model summary: summary Download '''AuReMe''' Input/Output [LI...") |
(Created page with "== SJAPGEM description == ''Saccharina japonica'' is the most cultivated alga worldwide, with nearly 8 millions tons of dry weight harvested in 2014, representing more than U...") |
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− | == | + | == SJAPGEM description == |
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+ | ''Saccharina japonica'' is the most cultivated alga worldwide, with nearly 8 millions tons of dry weight harvested in 2014, representing more than US $1.3 billion value ([http://www.fao.org/fishery/culturedspecies/Laminaria_japonica/en#tcNA008C Chen J., FAO website, accessed on january 10th, 2019]). | ||
+ | Its draft genome sequence was published in 2015 ([http://doi.org/10.1038/ncomms7986 Ye et al., Nature Communications]). The genome version used here underwent an additional round of cleaning for bacterial contaminants using Taxoblast ([https://doi.org/10.7717/peerj.4073 Dittami and Corre, 2017]). The paper describing this GEM reconstruction in more detail was published in 2019 ([https://doi.org/10.3390/antiox8110564 Nègre et al., Antioxidants]). In this study, extensive manual curation was performed on the carotenoid biosynthesis pathway (CAROTENOID-PWY) and on the first cycle of xanthophylls (PWY-5945). In a follow-up study (Girard et al., in preparation) we are now curating the sterol biosynthesis pathways (PWY-8191, PWY-8238). | ||
+ | |||
== Automatic reconstruction with [http://aureme.genouest.org AuReMe] == | == Automatic reconstruction with [http://aureme.genouest.org AuReMe] == | ||
Model summary: [[MEDIA:summary.txt|summary]] | Model summary: [[MEDIA:summary.txt|summary]] | ||
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Download '''AuReMe''' Input/Output [LINK OR MEDIA data] | Download '''AuReMe''' Input/Output [LINK OR MEDIA data] | ||
− | The automatic reconstruction of '' | + | The automatic reconstruction of ''Saccharina japonica'' results to a Genome scale [[MEDIA:S.japonica carotenoid curated.sbml|Model]] containing 3345 reactions, 2211 metabolites, 5021 genes and 1389 pathways. This GeM was obtained based on the following sources: |
+ | * Based on annotation data: | ||
+ | ** Tool: [http://bioinformatics.ai.sri.com/ptools/ Pathway tools] | ||
+ | *** Creation of a metabolic network containing 2552 reactions | ||
* Based on orthology data: | * Based on orthology data: | ||
− | ** | + | ** Tool: [http://pathtastic.gforge.inria.fr Pantograph] |
− | *** From template '' | + | *** From template ''NANNOCHLOROPSIS_SALINA'' ([https://doi.org/10.1186/s12918-017-0441-1 Loira et al., 2017]) creation of a metabolic network containing: 589 reactions |
− | *** From template '' | + | *** From template ''ARABIDOPSIS_THALIANA'' ([https://doi.org/10.1104/pp.109.148817 De Oliveira dal'Molin et al., 2010]) creation of a metabolic network containing: 419 reactions |
− | *** From template '' | + | *** From template ''[https://gem-aureme.genouest.org/esilgem/ ECTOCARPUS_SILICULOSUS]'' creation of a metabolic network containing: 1492 reactions |
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* Based on expertise: | * Based on expertise: | ||
− | ** | + | ** 64 reaction(s) added |
* Based on gap-filling: | * Based on gap-filling: | ||
− | *** | + | ** Tool: [https://pypi.python.org/pypi/meneco meneco] |
+ | *** 106 reaction(s) added | ||
− | + | [[FILE:venn.png|frameless|border]] | |
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== Collaborative curation == | == Collaborative curation == | ||
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** Download this [[MEDIA:Reaction_creator.csv|form]] | ** Download this [[MEDIA:Reaction_creator.csv|form]] | ||
* '''Follow the examples given in the form(s) to correctly share your suggestions''' | * '''Follow the examples given in the form(s) to correctly share your suggestions''' | ||
− | * Send the filled form(s) to: | + | * Send the filled form(s) to: gabriel.markov@sb-roscoff.fr |
Revision as of 11:20, 15 January 2021
SJAPGEM description
Saccharina japonica is the most cultivated alga worldwide, with nearly 8 millions tons of dry weight harvested in 2014, representing more than US $1.3 billion value (Chen J., FAO website, accessed on january 10th, 2019). Its draft genome sequence was published in 2015 (Ye et al., Nature Communications). The genome version used here underwent an additional round of cleaning for bacterial contaminants using Taxoblast (Dittami and Corre, 2017). The paper describing this GEM reconstruction in more detail was published in 2019 (Nègre et al., Antioxidants). In this study, extensive manual curation was performed on the carotenoid biosynthesis pathway (CAROTENOID-PWY) and on the first cycle of xanthophylls (PWY-5945). In a follow-up study (Girard et al., in preparation) we are now curating the sterol biosynthesis pathways (PWY-8191, PWY-8238).
Automatic reconstruction with AuReMe
Model summary: summary
Download AuReMe Input/Output [LINK OR MEDIA data]
The automatic reconstruction of Saccharina japonica results to a Genome scale Model containing 3345 reactions, 2211 metabolites, 5021 genes and 1389 pathways. This GeM was obtained based on the following sources:
- Based on annotation data:
- Tool: Pathway tools
- Creation of a metabolic network containing 2552 reactions
- Tool: Pathway tools
- Based on orthology data:
- Tool: Pantograph
- From template NANNOCHLOROPSIS_SALINA (Loira et al., 2017) creation of a metabolic network containing: 589 reactions
- From template ARABIDOPSIS_THALIANA (De Oliveira dal'Molin et al., 2010) creation of a metabolic network containing: 419 reactions
- From template ECTOCARPUS_SILICULOSUS creation of a metabolic network containing: 1492 reactions
- Tool: Pantograph
- Based on expertise:
- 64 reaction(s) added
- Based on gap-filling:
- Tool: meneco
- 106 reaction(s) added
- Tool: meneco